vimentin英 ['vɪməntɪn] 美 ['vɪməntɪn]
1. 波形蛋白：目的探讨重组反义波形蛋白cDNA逆转录病毒重组质粒对体外培养损伤的星形胶质细胞(astrocyte,AST)波形蛋白(vimentin)表达的影响.方法采用体外培养AST划伤模型,设实验组及对照组,通过免疫荧光、RT-PCR、Western blot等方法,
2. 网格蛋白：网间细胞interplexiform cell | 网格蛋白vimentin | 网织红细胞reticulocyte
1. Results:(1)The cultured cells were positive for cytokeratin and negative for vimentin, indicating the absence of contaminating elements and the cell viability evaluated by trypan blue exclusion was greater than 95%; (2)The levels of VEGF in the cell extracts and the VEGF mRNA of the cultured cells all increased gradually as the culturing time continuted during the 72hours'cultured time.
结果：(1)免疫组化技术证实培养细胞角蛋白阳性，波形蛋白阴性，纯度达95%以上；(2)正常培养的滋养细胞在培养12h后分泌产生的VEGF明显增加，至培养结束时的72h达到最高点；缺氧诱导滋养细胞大量分泌产生VEGF的时间提前至缺氧培养后6h，持续至72h无下降，VEGF水平明显高于正常，最高达到2200ng/ml，较正常上升47%；(3)滋养细胞VEGF mRNA表达于培养12h时开始明显上升，至48h时达峰值；缺氧则使VEGF mRNA表达显著上升的时间提前至6h，约12h达峰值。
2. Vimentin expressed predominantly in the granule cell layer near the ventricle in adult.
3. Our data showed that treatment with reveratrol inhibited cell mobility and up-regulation of the expression of E-cadherin, an epithelial marker, we also found that treatment with resveratrol down-regulating several mesenchymal markers such as fibronectin, N-cadherin and vimentin in various lung cancer cells.
4. The expression of vimentin in testis was detected by immunohistochemistry method.
5. Vimentin expression has some kinds of correlations with skeletal muscle cell proliferation, but it is not the only factor that matters.
6. Besides, vimentin expression was correlated with in vitro cell migration.
7. The passaged cells were typacl fibroblast morphous under inverted phase contrast microscope and showed immunocytochemical staining positively for Vimentin.
8. A dense perinuclear layer of vimentin fluorescence in erythroblast were observable throughout the cultural periods of 12, 24 and 36 hours.
9. Results Three cases of PB were biphasic type and were composed of primitive epithelial component and primitive stromal component; one case of PB was epithelial type and was shown as branching glands with clustered and well-differentiated structure. Immunohistochemical studies showed that AE1/AE3 was positive in the epithelial component in four cases, Vimentin was positive in the stromal component in three cases, thyroid transricption factor-1 was positive in two cases, CD117 was positive in one case, Ki-67 was 2% positive in one case, while P53 was negative in all cases. Conclusions The adult type PB is a rarely seen biphasic tumor.
10. The 58? kD protein reacts with anti-vimentin antibody and locates near the aboral wreath cilia.
11. Results: In comparison with the shame group, the protein levels ofα-SMA, Vimentin of UUO mice and treatment groups increased significantly, meanwhile Tripterygium Wlilfordii and ACEI could significantly reduce the expression ofα-SMA, Vimentin on levels of protein.
12. The characteristic of pathohistology was a changeable tumor, diagnosed by pigment granule in kytoplasm of neoplastic cell, HMB 45, S-100, and vimentin need further diagnosis.
13. The amount of vimentin IF per cell was higher than those of tubulin and F-actin at 12-24h of culture, but it broken down and decreased steadily when the cells became differentiated into late erythroblast at 36-48h of culture, thus facilitated the eccentric nucleation which we regard it as the initial step of denucleation. Both of the fluorescence intensity of tubulin and actin exhibited a significant rise and aggregated between the extruding nucleus and incipient reticulocyte before and during denucleation, and finally play a role in the commitent to ennucleation, the second phase of denucleation.
14. Results The results showed that positive rate of PCNA expression in 17 autopsy renal tissues was 64.71％; proliferative index of collecting tubes was significantly higher than that of distal convoluted tubules(P∨ 0.001 Vimentin expression of epithelial cells in distal convoluted tubules and collecting tubules from the EHF patients at hypotensive shock stage were strongly positive, PCNA expression at the same position was also positive, and positive intensity of the PCNA expression was consistent with severity of local lesions.
结果 EHF尸检肾组织PCNA的阳性率为64.71％，集合管的增殖指数显著大于远曲小管(P∨ 0.01)，低血压休克期患者远曲小管和集合管上皮细胞vimentin呈强阳性表达，相同部位 PCNA也呈阳性，PCNA的阳性强度与局部病变的程度相一致。
15. Fourfold increase of p53 expression in nuclei of spermatogonia and leydig's cells and the collaps of vimentin filaments in sertoli's cells were also found.
16. Methods The morphological characteristics of seminiferous tubule, vimentin filaments and p53 gene expression in sertoli's cells of adult SD rats orally exposed to 0.5%bisphenol-A were observed and analyzed by histo-chemical and immuno-histochemical methods.
17. Results There were characterized the tumor presentedin mass, cord and nests of epithelioid endothelial cells in hyaline myxoid matrix.
18. Results There were characterized the tumor presentedin mass, cord and nests of epithelioid endothelial cells in hyaline myxoid matrix. Immunohistochemically, tumor cell expressed vimentin CD34, CD31.
19. Expression of ΔNp63 in cultured cells was detected using immunohistochemistry and vimentin, and cytokeratin expressions were assessed by flow cytometric analysis.
20. In the pilocytic and gemistocytic astroytomas, the three markers all present strong positive, and along with the increase of astrocytomas degree, the intensity of GFAP and S-100proˉtein decreases, while the intensity of Vimentin protein increases. There is definite correlation between the quantity of the markers and the malignancy degree in the astrocytomas.